RT Journal Article SR Electronic T1 In vitro propagation of Trillium species JF Native Plants Journal JO NATIVE PLANTS JOURNAL FD University of Wisconsin Press SP 56 OP 63 DO 10.3368/npj.13.1.56 VO 13 IS 1 A1 Gagliardo, Ronald A1 Labarthe, Merrily A1 Zaic, Michael A1 Cruse-Sanders, Jennifer A1 Determann, Ronald O YR 2012 UL http://npj.uwpress.org/content/13/1/56.abstract AB For decades, trilliums (Liliaceae) have been collected from the wild for their beauty and medicinal properties. As a potential way to help alleviate the pressure from collecting and to advance the science of micropropagation, we examined past methods of in vitro propagation of Trillium and conducted our own preliminary experiments. We report our observations on the establishment of sterile cultures of Trillium persistens Duncan and T. reliquum J.D. Freeman along with further observations on root formation, cleaning, and media composition. We promote the use of in vitro techniques in the propagation of trilliums and recommend their application to the conservation of species that are threatened by collecting or habitat loss.