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Research ArticlePropagation Protocol
Open Access

Micropropagation of New Jersey tea (Ceanothus americanus), sand cherry (Prunus pumila), and sweetbells (Eubotrys racemosa)

Jessica D Lubell-Brand and Mark H Brand
Native Plants Journal, September 2021, 22 (3) 355-363; DOI: https://doi.org/10.3368/npj.22.3.355
Jessica D Lubell-Brand
Department of Plant Science and Landscape Architecture University of Connecticut 1376 Storrs Road, Unit-4067 Storrs, CT 06279
Roles: Associate Professor
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  • For correspondence: jessica.lubell{at}uconn.edu
Mark H Brand
Department of Plant Science and Landscape Architecture University of Connecticut 1376 Storrs Road, Unit-4067 Storrs, CT 06279
Roles: Professor
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  • For correspondence: mark.brand{at}uconn.edu
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    Figure 1.

    Summer inflorescences and low, mounded habit of New Jersey tea (Ceanothus americanus L. [Rhamnaceae]).

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    Figure 2.

    Copious flowers on sand cherry (Prunus pumila L. [Rosaceae]).

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    Figure 3.

    Urn-shaped flowers of sweetbells (Eubotrys racemosa L. [Ericaceae]).

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    Figure 4.

    Scarlet red fall foliage color on sweetbells.

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    Figure 5.

    Micropropagation of New Jersey tea: recently initiated shoots (A); proliferating shoots (B); rooted and acclimated microcuttings ready for transfer to the greenhouse (C); rooted microcuttings exhibiting a well-developed, coarse, white root system (D); fully acclimated growing plantlets from a 96-plug tray in the greenhouse ready for up-potting and transfer to outdoor conditions (E); high-quality liner plants in 106-ml pots (F).

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    Figure 6.

    Micropropagation of sand cherry: proliferating in vitro shoots (A); microcuttings at time of sticking (B); rooted and acclimated microcuttings ready for transfer to the greenhouse (C); high-quality liner plants in 307-ml pots (D).

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    Figure 7.

    Micropropagation of sweetbells: proliferating shoots (A); microcuttings at time of sticking (B); rooted and acclimated microcuttings ready for transfer to the greenhouse (C); rooted microcuttings exhibiting a fine root system (D); fully acclimated plantlets from a 96-plug tray in the greenhouse ready for up-potting and transfer to outdoor conditions (E); high-quality liner plants in 106-ml pots (F).

Tables

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    TABLE 1

    Micropropagation medium, shoot multiplication rate, and subculture frequency for in vitro cultures of New jersey tea, sand cherry, and sweetbells.

    SpeciesMedium typezHormoneySequestrene 330PHShoot multiplication rateSubculture frequency (d)
    New Jersey teaWP4 mg/l ZT50 mg/l5.22x21
    Sand cherryMS0.5 mg/l BA—5.78x28-35
    SweetbellsWP4 mg/l ZT50 mg/l5.23x35-42
    • ↵z Medium type abbreviations: Woody plant (WP), Murashige and Skoog with vitamins (MS).

    • ↵y Hormone abbreviations: zeatin (ZT), benzyladenine (BA).

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Native Plants Journal: 22 (3)
Native Plants Journal
Vol. 22, Issue 3
21 Sep 2021
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Micropropagation of New Jersey tea (Ceanothus americanus), sand cherry (Prunus pumila), and sweetbells (Eubotrys racemosa)
Jessica D Lubell-Brand, Mark H Brand
Native Plants Journal Sep 2021, 22 (3) 355-363; DOI: 10.3368/npj.22.3.355

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Micropropagation of New Jersey tea (Ceanothus americanus), sand cherry (Prunus pumila), and sweetbells (Eubotrys racemosa)
Jessica D Lubell-Brand, Mark H Brand
Native Plants Journal Sep 2021, 22 (3) 355-363; DOI: 10.3368/npj.22.3.355
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    • CURRENT STATE OF PROPAGATION
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Keywords

  • tissue culture
  • native shrubs
  • landscape plants
  • micropropagation
  • Rhamnaceae
  • Rosaceae
  • Ericaceae
  • USDA NRCS (2020)
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