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Research ArticleRefereed Research
Open Access

Development of a vegetative propagation protocol for Asclepias tuberosa

Mary Lewis, Matthew Chappell, Paul A Thomas, Donglin Zhang and Ockert Greyvenstein
Native Plants Journal, March 2020, 21 (1) 27-34; DOI: https://doi.org/10.3368/npj.21.1.27
Mary Lewis
Graduate Student, University of Georgia, Department of Horticulture, 1111 Miller Plant Science Building, Athens, GA 30602,
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  • For correspondence: snowbery{at}uga.edu
Matthew Chappell
Associate Professor, University of Georgia, Department of Horticulture, 1111 Miller Plant Science Building, Athens, GA 30602,
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  • For correspondence: hortprod{at}uga.edu
Paul A Thomas
Professor, University of Georgia, Department of Horticulture, 1111 Miller Plant Science Building, Athens, GA 30602,
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  • For correspondence: pathomas{at}uga.edu
Donglin Zhang
Professor University of Georgia, Department of Horticulture, 1111 Miller Plant Science Building, Athens, GA 30602,
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  • For correspondence: donglin{at}uga.edu
Ockert Greyvenstein
Plant Breeder, , Ball Horticultural Company, 400 Obispo Street, Guadalupe, CA 93434
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  • For correspondence: OGreyvenstein{at}ballhort.com
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  • Photo of a propagule at the midpoint of the study (4 wk after striking). Cuttings were initially 3 nodes in length and frequently resumed growth via axillary bud break or re-initiating growth from apical meristem while in the propagation environment.
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    Figure 1.

    Photo of a propagule at the midpoint of the study (4 wk after striking). Cuttings were initially 3 nodes in length and frequently resumed growth via axillary bud break or re-initiating growth from apical meristem while in the propagation environment.

  • Photo of cutting block taken 3 wk after cuttings were struck.
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    Figure 2.

    Photo of cutting block taken 3 wk after cuttings were struck.

  • Photo of cutting block taken 8 wk after cuttings were struck, at the time destructive harvesting occurred.
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    Figure 3.

    Photo of cutting block taken 8 wk after cuttings were struck, at the time destructive harvesting occurred.

  • Effect of cutting maturity on rooting success with K-IBA. With 25 successful cuttings possible per repetition, comparisons among hormone concentrations and maturity are displayed. Lighter bars (left) indicate all juvenile cuttings at differing K-IBA (potassium salt of indole-3-butyric acid) concentrations. Dark bars (right) indicate mature cuttings at differing K-IBA concentrations. Cutting age is defined as propagules harvested from 2-y-old stock plants 6 wk after breaking dormancy (M: mature) and cuttings harvested from seedlings 14 wk after germination (J: juvenile). Bars represent means with standard error based on standard one-way ANOVA. Treatment groups (columns) not sharing a letter are significantly different at P = 0.05 level based on adjusted P values using Tukey’s HSD.
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    Figure 4.

    Effect of cutting maturity on rooting success with K-IBA. With 25 successful cuttings possible per repetition, comparisons among hormone concentrations and maturity are displayed. Lighter bars (left) indicate all juvenile cuttings at differing K-IBA (potassium salt of indole-3-butyric acid) concentrations. Dark bars (right) indicate mature cuttings at differing K-IBA concentrations. Cutting age is defined as propagules harvested from 2-y-old stock plants 6 wk after breaking dormancy (M: mature) and cuttings harvested from seedlings 14 wk after germination (J: juvenile). Bars represent means with standard error based on standard one-way ANOVA. Treatment groups (columns) not sharing a letter are significantly different at P = 0.05 level based on adjusted P values using Tukey’s HSD.

  • Effect of cutting maturity on rooting success with NAA. With 25 successful cuttings possible per repetition, comparisons among hormone concentrations and maturity are displayed. Lighter bars (left) indicate all juvenile cuttings at differing NAA (laboratory grade, 1-naphthaleneacetic acid) concentrations. Dark bars (right) indicate mature cuttings at differing NAA concentrations. Cutting age is defined as propagules harvested from 2-y-old stock plants 6 wk after breaking dormancy (M: mature) and cuttings harvested from seedlings 14 wk after germination (J: juvenile). Bars represent means with standard error based on standard one-way ANOVA. Treatment groups (columns) not sharing a letter are significantly different at P = 0.05 level based on adjusted P values using Tukey’s HSD.
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    Figure 5.

    Effect of cutting maturity on rooting success with NAA. With 25 successful cuttings possible per repetition, comparisons among hormone concentrations and maturity are displayed. Lighter bars (left) indicate all juvenile cuttings at differing NAA (laboratory grade, 1-naphthaleneacetic acid) concentrations. Dark bars (right) indicate mature cuttings at differing NAA concentrations. Cutting age is defined as propagules harvested from 2-y-old stock plants 6 wk after breaking dormancy (M: mature) and cuttings harvested from seedlings 14 wk after germination (J: juvenile). Bars represent means with standard error based on standard one-way ANOVA. Treatment groups (columns) not sharing a letter are significantly different at P = 0.05 level based on adjusted P values using Tukey’s HSD.

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    TABLE 1

    Effect of hormone type (K-IBA or NAA) and concentration on the mean number of roots, root length, and plant height at harvest (8 wk after striking).

    TreatmentMature cuttings
    Mean number of rootsMean root lengthMean plant height
    Control9.19±1.32 ab9.43±0.82 a3.71±0.37 c
    K-IBA-1000 ppm7.5±1.35 ab8.5±0.89 a6.2±0.41 ab
    K-IBA-3000 ppm11.94±1.35 a8.34±0.91 a7.94±0.42 a
    K-IBA-5000 ppm11.1±1.35 ab7.79±0.86 a3.46±0.39 c
    K-IBA-8000 ppm9.52±1.35 ab8.15±0.86 a4.4±0.39 bc
    K-IBA-50 ppm———
    NAA-500 ppm5.75±1.35 b8.18±0.84 a4.74±0.38 bc
    NAA-1000 ppm8.52±1.35 ab5.75±0.91 a.28±0.42 c
    NAA-1500 ppm9.87±1.35 ab6.5±0.94 a4.11±0.43 c
    NAA-2000 ppm10.35±1.35 ab8.11±0.84 a4.23±0.38 c
    Significancea0.02120.05< 0.001
    • Notes: Results depicted apply only to propagules collected from mature (2-y-old) stock plants collected and struck 6 wk after breaking dormancy yet prior to floral initiation. All values are reported in cm: 1 cm = 0.4 in. Standard error of the means are reported in addition to means. K-IBA = indole-3-butyric acid; NAA = 1-naphthaleneacetic acid.

    • ↵a Significance of treatment effects (P > F; ns = not significant at the 0.05 level). Means in same treatment group (columns) not sharing a letter are significantly different at P < 0.05 level based on adjusted P values using Tukey HSD and a standard one-way ANOVA.

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Native Plants Journal: 21 (1)
Native Plants Journal
Vol. 21, Issue 1
20 Mar 2020
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Development of a vegetative propagation protocol for Asclepias tuberosa
Mary Lewis, Matthew Chappell, Paul A Thomas, Donglin Zhang, Ockert Greyvenstein
Native Plants Journal Mar 2020, 21 (1) 27-34; DOI: 10.3368/npj.21.1.27

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Development of a vegetative propagation protocol for Asclepias tuberosa
Mary Lewis, Matthew Chappell, Paul A Thomas, Donglin Zhang, Ockert Greyvenstein
Native Plants Journal Mar 2020, 21 (1) 27-34; DOI: 10.3368/npj.21.1.27
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Keywords

  • cutting propagation
  • native plant
  • butterfly weed
  • Apocynaceae
  • Asclepiadaceae
  • indole-3-butyric acid
  • 1-naphthaleneacetic acid
  • Plants: University of Georgia Center for Invasive Species and Ecosystem Health (2019)
  • Plants: USDA NRCS (2019)
  • Insects: ITIS (2019)
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